{"id":793,"date":"2014-12-23T17:26:55","date_gmt":"2014-12-23T17:26:55","guid":{"rendered":"http:\/\/cmed.lab.mcgill.ca\/?p=793"},"modified":"2014-12-23T18:38:07","modified_gmt":"2014-12-23T18:38:07","slug":"high-throughput-screening-now-3d","status":"publish","type":"post","link":"https:\/\/moraeslab.com\/cmed\/high-throughput-screening-now-3d\/","title":{"rendered":"High-throughput screening&#8230; now in 3D!"},"content":{"rendered":"<p>We know that 3D cultures can be more realistic than growing cells on hard, flat 2D surfaces. \u00a0However,\u00a0conducting high-throughput screens in 3D is extremely challenging from a technical, logistical and cost perspective. \u00a0To address these\u00a0issues, Brendan and Chris developed a micro-scale 3D culture assay that can be integrated directly into existing high-throughput screening infrastructure, is only slightly more expensive than conventional 2D assays, and eliminates the transport limitations typically associated with 3D systems. \u00a0A preliminary screen of breast cancer cell chemo-responsiveness showed dramatic differences in chemotherapeutic activity\u00a0between 2D and 3D cultures: \u00a0check out the paper <a href=\"http:\/\/moraeslab.com\/cmed\/wp-content\/uploads\/2013\/11\/23_JALA_3Dhts.pdf\" target=\"_blank\">here<\/a>\u00a0and on our <a title=\"Publications\" href=\"http:\/\/moraeslab.com\/cmed\/publications\/\">Publications <\/a>page.<\/p>\n  <div class=\"otw-row\"><\/div>","protected":false},"excerpt":{"rendered":"<p>We know that 3D cultures can be more realistic than growing cells on hard, flat 2D surfaces. \u00a0However,\u00a0conducting high-throughput screens in 3D is extremely challenging from a technical, logistical and cost perspective. \u00a0To address these\u00a0issues, Brendan and Chris developed a<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"class_list":["post-793","post","type-post","status-publish","format-standard","hentry","category-news"],"_links":{"self":[{"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/posts\/793","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/comments?post=793"}],"version-history":[{"count":0,"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/posts\/793\/revisions"}],"wp:attachment":[{"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/media?parent=793"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/categories?post=793"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/moraeslab.com\/cmed\/wp-json\/wp\/v2\/tags?post=793"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}